Review



mouse testis cdna library  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    TaKaRa mouse testis cdna library
    Mouse Testis Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+cdna/Mouse+Testis+QUICK-Clone+cDNA/bio_rxiv__2025__05__28__656576-259-15-24
    Average 93 stars, based on 250 article reviews
    mouse testis cdna library - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes.
    Article Snippet: Cell Biochem Funct.. 2018;1–11.. While transcription plays an obviously important role in gene expression, translation has recently been emerged as a key step that defines the composition and quality of the proteome in the cell of higher eukaryotes including mammals.

    Article Title: Mouse Class III myosins: Kinase activity and phosphorylation sites
    Article Snippet: .. Construction of recombinant baculovirus, expression, and purification of the mouse myosin III kinases The mMyo3B kinase/transition domains were amplified from plasmid containing a cDNA clone encoding full length Myo3B ( Katti et al., 2009 ); the mMyo3A kinase/transition domains were amplified from mouse testis cDNA (Clontech, Mountain View, CA). ..

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: For 5’ RACE, 4 μg of total RNA from testes was reversetranscribed using 5’ RACE System for Rapid Amplification of cDNA Ends (Invitrogen, 18374058) and synthesized cDNA was PCR-amplified, cloned into the pCR4-TOPO TA vector (Invitrogen, K457501) and Sanger-sequenced to identify translation initiation site. .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S). .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S).

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis.
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Article Title: Involvement of <scp>GLCCI1</scp> in mouse spermatogenesis
    Article Snippet: © 2022 Federation of American Societies for Experimental Biology.. Abbreviations: 5′UTR, five prime untranslated region; Ab, antibody; AR, androgen receptor; Bim, Bcl2l11; Ccasp3, cleavedcaspase3; CAGE, cap analysis of gene expression; cDNA, complementary deoxyribonucleic acid; CNBr, cyanogen bromide; DAPI, 4′,6diamidino2phenylindole; DEX, dexamethasone; DMEM, Dulbecco's modified Eagle's medium; EDTA, ethylenediaminetetraacetic acid; ER, estrogen receptor; FBS, fetal bovine serum; FSH, folliclestimulating hormone; GC, glucocorticoid; GLCCI1, glucocorticoidinduced transcript1; GR, glucocorticoid receptor; mRNA, messenger ribonucleic acid; HEK293, human embryonic kidney cell 293; HRP, horseradish peroxidase; IgG, immunoglobulin G; kb, kilo base; kDa, kilodalton; LC8, dynein light chain LC8type 1; MEMFA, MOPS EGTA magnesium sulfate formaldehyde buffer; PAK1, p21protein activated kinase 1; PBS, phosphate buffer saline; PCR, polymerase chain reaction; PMSF, phenylmethylsulfonyl fluoride; RTPCR, reverse transcription polymerase chain reaction; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; TBS, trisbuffered saline; tRNA, transfer ribonucleic acid; UTP, uridine triphosphate.. 1Department of Pediatrics, Kyorin University School of Medicine, Tokyo, Japan 2Department of Microscopic Anatomy, Kyorin University School of Medicine, Tokyo, Japan 3Molecular Medicine Center and Department of Medical Chemistry and Biochemistry, Medical University of Sofia, Sofia, Bulgaria 4Diagnostics and Therapeutics of Intractable Diseases, Intractable Disease Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan 5Department of Toxicology and Pharmacology, Kyorin University School of Medicine, Tokyo, Japan 6Department of Molecular Cell Biology, Faculty of Medical Technology, Graduate School of Health Sciences, Fujita Health University, Toyoake, Japan

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: .. Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7- Rnf212b C9A , pGADT7- Rnf212 C10A , and pGBKT7- Rnf212b C9A vectors were generated using QuickChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513). .. Y187 and Y2HGold yeast two-hybrid strains were transformed with vectors containing genes of interest or alone (empty vectors), mated on SD-Trp-Leu plates, and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech).

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Polymerase Chain Reaction:

    Article Title: The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes.
    Article Snippet: Cell Biochem Funct.. 2018;1–11.. While transcription plays an obviously important role in gene expression, translation has recently been emerged as a key step that defines the composition and quality of the proteome in the cell of higher eukaryotes including mammals.

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: For 5’ RACE, 4 μg of total RNA from testes was reversetranscribed using 5’ RACE System for Rapid Amplification of cDNA Ends (Invitrogen, 18374058) and synthesized cDNA was PCR-amplified, cloned into the pCR4-TOPO TA vector (Invitrogen, K457501) and Sanger-sequenced to identify translation initiation site. .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S). .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S).

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis.
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Article Title: Involvement of <scp>GLCCI1</scp> in mouse spermatogenesis
    Article Snippet: © 2022 Federation of American Societies for Experimental Biology.. Abbreviations: 5′UTR, five prime untranslated region; Ab, antibody; AR, androgen receptor; Bim, Bcl2l11; Ccasp3, cleavedcaspase3; CAGE, cap analysis of gene expression; cDNA, complementary deoxyribonucleic acid; CNBr, cyanogen bromide; DAPI, 4′,6diamidino2phenylindole; DEX, dexamethasone; DMEM, Dulbecco's modified Eagle's medium; EDTA, ethylenediaminetetraacetic acid; ER, estrogen receptor; FBS, fetal bovine serum; FSH, folliclestimulating hormone; GC, glucocorticoid; GLCCI1, glucocorticoidinduced transcript1; GR, glucocorticoid receptor; mRNA, messenger ribonucleic acid; HEK293, human embryonic kidney cell 293; HRP, horseradish peroxidase; IgG, immunoglobulin G; kb, kilo base; kDa, kilodalton; LC8, dynein light chain LC8type 1; MEMFA, MOPS EGTA magnesium sulfate formaldehyde buffer; PAK1, p21protein activated kinase 1; PBS, phosphate buffer saline; PCR, polymerase chain reaction; PMSF, phenylmethylsulfonyl fluoride; RTPCR, reverse transcription polymerase chain reaction; SD, standard deviation; SDSPAGE, sodium dodecyl sulfatepolyacrylamide gel electrophoresis; TBS, trisbuffered saline; tRNA, transfer ribonucleic acid; UTP, uridine triphosphate.. 1Department of Pediatrics, Kyorin University School of Medicine, Tokyo, Japan 2Department of Microscopic Anatomy, Kyorin University School of Medicine, Tokyo, Japan 3Molecular Medicine Center and Department of Medical Chemistry and Biochemistry, Medical University of Sofia, Sofia, Bulgaria 4Diagnostics and Therapeutics of Intractable Diseases, Intractable Disease Research Center, Juntendo University Graduate School of Medicine, Tokyo, Japan 5Department of Toxicology and Pharmacology, Kyorin University School of Medicine, Tokyo, Japan 6Department of Molecular Cell Biology, Faculty of Medical Technology, Graduate School of Health Sciences, Fujita Health University, Toyoake, Japan

    Article Title: Upregulation of cellular glutathione levels in human ABCB5 - and murine Abcb5 -transfected cells
    Article Snippet: .. Murine Abcb5 cDNA (GenBank ID: NM_029961) was isolated by PCR using mouse testis cDNA (Takara, Ohtsu, Japan) as a template. ..

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: .. Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7- Rnf212b C9A , pGADT7- Rnf212 C10A , and pGBKT7- Rnf212b C9A vectors were generated using QuickChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513). .. Y187 and Y2HGold yeast two-hybrid strains were transformed with vectors containing genes of interest or alone (empty vectors), mated on SD-Trp-Leu plates, and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech).

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Clone Assay:

    Article Title: The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes.
    Article Snippet: Cell Biochem Funct.. 2018;1–11.. While transcription plays an obviously important role in gene expression, translation has recently been emerged as a key step that defines the composition and quality of the proteome in the cell of higher eukaryotes including mammals.

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: For 5’ RACE, 4 μg of total RNA from testes was reversetranscribed using 5’ RACE System for Rapid Amplification of cDNA Ends (Invitrogen, 18374058) and synthesized cDNA was PCR-amplified, cloned into the pCR4-TOPO TA vector (Invitrogen, K457501) and Sanger-sequenced to identify translation initiation site. .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S). .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S).

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis.
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: .. Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7- Rnf212b C9A , pGADT7- Rnf212 C10A , and pGBKT7- Rnf212b C9A vectors were generated using QuickChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513). .. Y187 and Y2HGold yeast two-hybrid strains were transformed with vectors containing genes of interest or alone (empty vectors), mated on SD-Trp-Leu plates, and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech).

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Recombinant:

    Article Title: Mouse Class III myosins: Kinase activity and phosphorylation sites
    Article Snippet: .. Construction of recombinant baculovirus, expression, and purification of the mouse myosin III kinases The mMyo3B kinase/transition domains were amplified from plasmid containing a cDNA clone encoding full length Myo3B ( Katti et al., 2009 ); the mMyo3A kinase/transition domains were amplified from mouse testis cDNA (Clontech, Mountain View, CA). ..

    Expressing:

    Article Title: Mouse Class III myosins: Kinase activity and phosphorylation sites
    Article Snippet: .. Construction of recombinant baculovirus, expression, and purification of the mouse myosin III kinases The mMyo3B kinase/transition domains were amplified from plasmid containing a cDNA clone encoding full length Myo3B ( Katti et al., 2009 ); the mMyo3A kinase/transition domains were amplified from mouse testis cDNA (Clontech, Mountain View, CA). ..

    Purification:

    Article Title: Mouse Class III myosins: Kinase activity and phosphorylation sites
    Article Snippet: .. Construction of recombinant baculovirus, expression, and purification of the mouse myosin III kinases The mMyo3B kinase/transition domains were amplified from plasmid containing a cDNA clone encoding full length Myo3B ( Katti et al., 2009 ); the mMyo3A kinase/transition domains were amplified from mouse testis cDNA (Clontech, Mountain View, CA). ..

    Plasmid Preparation:

    Article Title: Mouse Class III myosins: Kinase activity and phosphorylation sites
    Article Snippet: .. Construction of recombinant baculovirus, expression, and purification of the mouse myosin III kinases The mMyo3B kinase/transition domains were amplified from plasmid containing a cDNA clone encoding full length Myo3B ( Katti et al., 2009 ); the mMyo3A kinase/transition domains were amplified from mouse testis cDNA (Clontech, Mountain View, CA). ..

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis.
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Article Title: Prokineticin 2 Plays a Pivotal Role in Psoriasis
    Article Snippet: .. The coding region of mouse PK2 was amplified from mouse testis cDNA by standard PCR and cloned into the EcoRI and BamHI site of PLVX-puro vector (Clontech Laboratories, Inc., USA). ..

    Cloning:

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: For 5’ RACE, 4 μg of total RNA from testes was reversetranscribed using 5’ RACE System for Rapid Amplification of cDNA Ends (Invitrogen, 18374058) and synthesized cDNA was PCR-amplified, cloned into the pCR4-TOPO TA vector (Invitrogen, K457501) and Sanger-sequenced to identify translation initiation site. .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S). .. Full-length mouse Rnf212b and Rnf212 cDNAs were PCR amplified from mouse testis cDNA, prepared as above, and cloned into pGADT7 and pGBKT7 vectors (Clontech) using a Gibson Assembly Cloning Kit (NEB, E2611S).

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: .. Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7- Rnf212b C9A , pGADT7- Rnf212 C10A , and pGBKT7- Rnf212b C9A vectors were generated using QuickChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513). .. Y187 and Y2HGold yeast two-hybrid strains were transformed with vectors containing genes of interest or alone (empty vectors), mated on SD-Trp-Leu plates, and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech).

    Isolation:

    Article Title: Upregulation of cellular glutathione levels in human ABCB5 - and murine Abcb5 -transfected cells
    Article Snippet: .. Murine Abcb5 cDNA (GenBank ID: NM_029961) was isolated by PCR using mouse testis cDNA (Takara, Ohtsu, Japan) as a template. ..

    Generated:

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: .. Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7- Rnf212b C9A , pGADT7- Rnf212 C10A , and pGBKT7- Rnf212b C9A vectors were generated using QuickChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513). .. Y187 and Y2HGold yeast two-hybrid strains were transformed with vectors containing genes of interest or alone (empty vectors), mated on SD-Trp-Leu plates, and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech).

    Mutagenesis:

    Article Title: Distinct and interdependent functions of three RING proteins regulate recombination during mammalian meiosis
    Article Snippet: .. Full-length mouse Rnf212b and Rnf212 cDNAs were amplified by PCR from mouse testis cDNA prepared as above and cloned into pGADT7 and pGBKT7 vectors (Clontech) using the Gibson Assembly Cloning Kit (NEB, E2611S). pGADT7- Rnf212b C9A , pGADT7- Rnf212 C10A , and pGBKT7- Rnf212b C9A vectors were generated using QuickChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, 210513). .. Y187 and Y2HGold yeast two-hybrid strains were transformed with vectors containing genes of interest or alone (empty vectors), mated on SD-Trp-Leu plates, and selection was performed on SD-Trp-Leu plates containing 200 ng/ml of Aureobasidin A (Clontech, 630466) according to the manufacturer’s instructions (Clontech).



    Similar Products

    93
    TaKaRa mouse testis cdna library
    Mouse Testis Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+cdna/Mouse+Testis+QUICK-Clone+cDNA/bio_rxiv__2025__05__28__656576-259-15-24
    Average 93 stars, based on 1 article reviews
    mouse testis cdna library - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    93
    TaKaRa mouse testis cdna
    Mouse Testis Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+cdna/Mouse+Testis+QUICK-Clone+cDNA/pmc11745341__pnas__2412961121__sapp-20-10-23
    Average 93 stars, based on 1 article reviews
    mouse testis cdna - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    95
    TaKaRa mouse sirt1 from a mouse testis cdna library
    (A) The Δ tdh1 Δ tdh2 Δ tdh3 strain MR173 containing the plasmid p( URA3 )- Eco GAP was transformed with the indicated HIS3 plasmids. Transformants were selected, grown overnight, and spotted in a five-fold serial dilution on SC media containing 5′FOA for counter-selection of the URA3 plasmid or on SC media as control. Plates were then incubated for 3 days at 30°C. (B) Similar experiment as described in (A), but using Δ rki1 yeast expressing the human RKI1 orthologue (Rpi1) from the URA3 plasmid. (C) Δ tdh1 Δ tdh2 Δ tdh3 yeast overexpressing Sir2 or its mammalian homologues, human <t>SirT1,</t> human SirT2, and mouse SirT2, were processed as in (A). (D) Similar to (C), but overexpressing the mutant proteins Sir2 H364Y and Sir2 P394L . (E) The yeast strain MR110, in which chromosomal TPI1 is deleted and yeast TPI1 is expressed from an URA3 episome, was transformed with the indicated HIS3 plasmids and processed as described above. (F) Similar experiment using the quadruple deletion strain Δ tdh1 Δ tdh2 Δ tdh3 Δ zwf1 . (G) Similar to (E), but using yeast strain MR101, which is isogenic to MR110, but expresses human instead of yeast Tpi1 from the URA3 episome.
    Mouse Sirt1 From A Mouse Testis Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+cdna/Mouse+MTC+Panel+III/pmc02670500-210-15-23
    Average 95 stars, based on 1 article reviews
    mouse sirt1 from a mouse testis cdna library - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    93
    TaKaRa mouse testis quick
    (A) The Δ tdh1 Δ tdh2 Δ tdh3 strain MR173 containing the plasmid p( URA3 )- Eco GAP was transformed with the indicated HIS3 plasmids. Transformants were selected, grown overnight, and spotted in a five-fold serial dilution on SC media containing 5′FOA for counter-selection of the URA3 plasmid or on SC media as control. Plates were then incubated for 3 days at 30°C. (B) Similar experiment as described in (A), but using Δ rki1 yeast expressing the human RKI1 orthologue (Rpi1) from the URA3 plasmid. (C) Δ tdh1 Δ tdh2 Δ tdh3 yeast overexpressing Sir2 or its mammalian homologues, human <t>SirT1,</t> human SirT2, and mouse SirT2, were processed as in (A). (D) Similar to (C), but overexpressing the mutant proteins Sir2 H364Y and Sir2 P394L . (E) The yeast strain MR110, in which chromosomal TPI1 is deleted and yeast TPI1 is expressed from an URA3 episome, was transformed with the indicated HIS3 plasmids and processed as described above. (F) Similar experiment using the quadruple deletion strain Δ tdh1 Δ tdh2 Δ tdh3 Δ zwf1 . (G) Similar to (E), but using yeast strain MR101, which is isogenic to MR110, but expresses human instead of yeast Tpi1 from the URA3 episome.
    Mouse Testis Quick, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+testis+cdna/Mouse+Testis+QUICK-Clone+cDNA/pmc02686705-123-15-19
    Average 93 stars, based on 1 article reviews
    mouse testis quick - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    (A) The Δ tdh1 Δ tdh2 Δ tdh3 strain MR173 containing the plasmid p( URA3 )- Eco GAP was transformed with the indicated HIS3 plasmids. Transformants were selected, grown overnight, and spotted in a five-fold serial dilution on SC media containing 5′FOA for counter-selection of the URA3 plasmid or on SC media as control. Plates were then incubated for 3 days at 30°C. (B) Similar experiment as described in (A), but using Δ rki1 yeast expressing the human RKI1 orthologue (Rpi1) from the URA3 plasmid. (C) Δ tdh1 Δ tdh2 Δ tdh3 yeast overexpressing Sir2 or its mammalian homologues, human SirT1, human SirT2, and mouse SirT2, were processed as in (A). (D) Similar to (C), but overexpressing the mutant proteins Sir2 H364Y and Sir2 P394L . (E) The yeast strain MR110, in which chromosomal TPI1 is deleted and yeast TPI1 is expressed from an URA3 episome, was transformed with the indicated HIS3 plasmids and processed as described above. (F) Similar experiment using the quadruple deletion strain Δ tdh1 Δ tdh2 Δ tdh3 Δ zwf1 . (G) Similar to (E), but using yeast strain MR101, which is isogenic to MR110, but expresses human instead of yeast Tpi1 from the URA3 episome.

    Journal: PLoS ONE

    Article Title: Interfering with Glycolysis Causes Sir2-Dependent Hyper-Recombination of Saccharomyces cerevisiae Plasmids

    doi: 10.1371/journal.pone.0005376

    Figure Lengend Snippet: (A) The Δ tdh1 Δ tdh2 Δ tdh3 strain MR173 containing the plasmid p( URA3 )- Eco GAP was transformed with the indicated HIS3 plasmids. Transformants were selected, grown overnight, and spotted in a five-fold serial dilution on SC media containing 5′FOA for counter-selection of the URA3 plasmid or on SC media as control. Plates were then incubated for 3 days at 30°C. (B) Similar experiment as described in (A), but using Δ rki1 yeast expressing the human RKI1 orthologue (Rpi1) from the URA3 plasmid. (C) Δ tdh1 Δ tdh2 Δ tdh3 yeast overexpressing Sir2 or its mammalian homologues, human SirT1, human SirT2, and mouse SirT2, were processed as in (A). (D) Similar to (C), but overexpressing the mutant proteins Sir2 H364Y and Sir2 P394L . (E) The yeast strain MR110, in which chromosomal TPI1 is deleted and yeast TPI1 is expressed from an URA3 episome, was transformed with the indicated HIS3 plasmids and processed as described above. (F) Similar experiment using the quadruple deletion strain Δ tdh1 Δ tdh2 Δ tdh3 Δ zwf1 . (G) Similar to (E), but using yeast strain MR101, which is isogenic to MR110, but expresses human instead of yeast Tpi1 from the URA3 episome.

    Article Snippet: Human Rpi1 and SirT1 coding sequences were amplified from a human fetal cDNA library (Clontech), mouse SirT1 from a mouse testis cDNA library (Clontech), K. lactis GDP1 from p1696 , SIR2 from pAR14 , Hst2 from BY4741-, and Eco GAP and lac Z from E. coli genomic DNA (strains Xl1blue and GM2929, respectively).

    Techniques: Plasmid Preparation, Transformation Assay, Serial Dilution, Selection, Incubation, Expressing, Mutagenesis

    Plasmids used in this study.

    Journal: PLoS ONE

    Article Title: Interfering with Glycolysis Causes Sir2-Dependent Hyper-Recombination of Saccharomyces cerevisiae Plasmids

    doi: 10.1371/journal.pone.0005376

    Figure Lengend Snippet: Plasmids used in this study.

    Article Snippet: Human Rpi1 and SirT1 coding sequences were amplified from a human fetal cDNA library (Clontech), mouse SirT1 from a mouse testis cDNA library (Clontech), K. lactis GDP1 from p1696 , SIR2 from pAR14 , Hst2 from BY4741-, and Eco GAP and lac Z from E. coli genomic DNA (strains Xl1blue and GM2929, respectively).

    Techniques: